MONITORING OF THE PARASITE LOAD IN THE DIGESTIVE TRACT OF RHODNIUS PROLIXUS BY COMBINED QPCR ANALYSIS AND IMAGING TECHNIQUES PROVIDES NEW INSIGHTS INTO THE TRYPANOSOME LIFE CYCLE.

Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle.

Here we report the monitoring of the digestive tract colonization of Rhodnius prolixus by Trypanosoma cruzi using an accurate determination of the parasite load by qPCR coupled with fluorescence and bioluminescence imaging (BLI).These complementary methods revealed critical steps necessary for the parasite population to colonize the insect gut and

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Transcriptome analysis reveals the mechanism of stromal cell-derived factor-1 and exendin-4 synergistically promoted periodontal ligament stem cells osteogenic differentiation

Stromal cell-derived factor-1 (SDF-1) and Exendin-4 (EX-4) play beneficial roles in promoting periodontal ligament stem cells (PDLSCs) osteogenic differentiation, while the detailed mechanism has not been clarified.In this study, we aimed to evaluate the biological mechanism of SDF-1 and EX-4 alone or synergistic application in regulating PDLSCs di

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